Eighth-tier Maldon & Tiptree will travel to League One Port Vale in the FA Cup first round while 2013 winners Wigan Athletic host Hemel Hempstead Town of the National League South.
Essex-based side Maldon & Tiptree are the lowest-ranked team remaining in the competition.
The Jammers currently lead the Isthmian League North Division - 103 places below third-tier Port Vale in the English football pyramid.
Meanwhile, new Luton Town boss Jack Wilshere will come up against Robbie Savage if Forest Green Rovers beat Worthing on Monday evening.
Former Arsenal and England midfielder Wilshere, 33, was named as Matt Bloomfield's successor at Kenilworth Road earlier on Monday.
Elsewhere, Tom Cleverley's Plymouth Argyle will take on Wycombe Wanderers at Home Park, with Huddersfield Town travelling to Bolton Wanderers in three all-League One ties.
Vertu Trophy holders Peterborough United are at home to Cardiff City, while Oldham Athletic host Northampton Town and Crewe Alexandra will face Doncaster Rovers.
Should seventh-tier Gainsborough United beat Hartlepool United in their fourth qualifying round replay, the Lincolnshire side will welcome League Two Accrington Stanley to The Northolme.
The ties will be played across the weekend of Saturday, 1 and Sunday, 2 November.
Holders Crystal Palace and the rest of the clubs playing in the Premier League and Championship will enter the competition in the third round.
Weston-super-Mare v Aldershot Town
Salford City v Lincoln City
Luton Town v Worthing or Forest Green Rovers
Gainsborough Trinity or Hartlepool United v Accrington Stanley
Colchester United v Milton Keynes Dons
Tranmere Rovers v Stockport County
Wigan Athletic v Hemel Hempstead Town
Newport County v Gillingham
Cheltenham Town v Bradford City
Barnsley v York City
Reading v Carlisle United
Bromley v Bristol Rovers
Peterborough United v Cardiff City
Oldham Athletic v Northampton Town
Crewe Alexandra v Doncaster Rovers
Tamworth v Leyton Orient
Stevenage v Chesterfield
Boreham Wood v Crawley Town
Farnham Town or Sutton United v AFC Telford United
Bolton Wanderers v Huddersfield Town
Chelmsford City v Braintree Town
Spennymoor Town v Barrow
Wycombe Wanderers v Plymouth Argyle
Halifax Town v Exeter City
Slough Town v Altrincham or Harborough Town
Wealdstone v Southend United
Rotherham United v Swindon Town
Grimsby Town v Ebbsfleet United
Buxton v Chatham Town
Burton Albion v Banbury United or St Albans City
Woking or Brackley Town v Notts County
Blackpool v Scunthorpe United
Cambridge United v Morecambe or Chester
AFC Wimbledon v Gateshead
Mansfield Town v Harrogate Town
Macclesfield v AFC Totton or Truro City
South Shields v Shrewsbury Town
Fleetwood Town v Barnet
Port Vale v Maldon & Tiptree
Eastleigh v Walsall