Last season Ipswich Town captain Sam Morsy did not wear the rainbow armband, while Crystal Palace captain Marc Guehi wrote "I love Jesus" on his.
Manchester United abandoned plans to wear a jacket supporting the LGBTQ+ campaign because one player refused to wear it.
"Premier League With Pride demonstrates our ongoing support for the LGBTQ+ community, and our commitment to ensuring that football is for everyone and inclusivity is embedded across all areas of the game," said Premier League chief executive Richard Masters.
"We want everyone involved in football to feel welcome, supported and comfortable being themselves."
Stonewall says it remains committed to continuing to work with Premier League clubs.
The league's new campaign is being launched in partnership with national LGBTQ+ support line Switchboard.
"There's optimism going into this week of matches and the Premier League is celebrating and talking about education as part of this activation," said Jon Holmes from Football v Homophobia.
"There's a really deep element to this and I think that's hopefully how this new initiative will evolve out of Rainbow Laces as well."
In 2021 the BBC opted to withdraw from a workplace equality scheme run by Stonewall after questions were raised over how the broadcaster could remain impartial over issues the charity was campaigning about.

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